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  #5,523,204
Detection of nucleic acids in cells by strand displacement amplification.
inventors: Robert H Singer, Jean Marie Mathys, Kenton L. Lohman
Filed on December 10, 1993, awarded on June 4, 1996.

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Strand Displacement Amplification for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues. Excellent specimen morphology is preserved, and either DNA targets, or RNA targets, or both may be selectively amplified. In situ amplification by SDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.
 
 
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