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| < previous patent | all patents | next patent > |
| #6,203,986 |
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A
method for visualizing the location and movement of a specific RNA of
interest in a living cell, in real time, is disclosed. The method includes
the following steps: (a) providing a DNA encoding the RNA, which RNA includes
a protein-binding site; (b) providing a nucleic acid encoding a fusion
protein, which fusion protein comprises a fluorescent domain and an RNA-binding
domain; (c) introducing the DNA encoding the RNA, and the nucleic acid
encoding the fusion protein, into a eukaryotic cell so that the DNA encoding
the RNA and the nucleic acid encoding the fusion protein are expressed
in the cell; and (d) detecting fluorescence outside the nucleus or inside
the nucleus of the cell, with the fluorescence being from the fusion protein
bound to the RNA. In some embodiments, the fusion protein also includes
an intracellular localization domain. |
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