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A key
goal of biology is to relate the expression of specific genes to a particular
cellular phenotype. However, current assays for gene expression destroy
the structural context. By combining advances in computational fluorescence
microscopy with multiplex probe design, we devised technology in which
the expression of many genes can be visualized simultaneously inside
single cells with high spatial and temporal resolution. Analysis of
11 genes in serum-stimulated cultured cells revealed unique patterns
of gene expression within individual cells. Using the nucleus as the
substrate for parallel gene analysis, we provide a platform for the
fusion of genomics and cell biology: "cellular genomics." |
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