Gene expression profiling in single cells within tissue.

Singer Lab Publications:

Abstract:

Gene expression profiling in single cells within tissue.

Capodieci P, Donovan M, Buchinsky H, Jeffers Y, Cordon-Cardo C, Gerald W, Edelson J, Shenoy SM, Singer RH

Nat Methods 2(9):663-665 (2005 September)

Link to Journal | Supplemental Online Information | Download Reprint (PDF)

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.