Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body.

Singer Lab Publications:

Abstract:

Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body.

Chang W, Zaarour RF, Reck-Peterson S, Rinn J, Singer RH, Snyder M, Novick P, Mooseker MS

RNA 14(3):491-502 (2008 March)

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Myo2p is an essential class V myosin in budding yeast with severalidentified functions in organelle trafficking and spindle orientation.The present study demonstrates that Myo2p is a component ofa large RNA-containing complex (Myo2p–RNP) that is distinctfrom polysomes based on sedimentation analysis and lack of ribosomalsubunits in the Myo2p–RNP. Microarray analysis of RNAsthat coimmunoprecipitate with Myo2p revealed the presence ofa large number of mRNAs in this complex. The Myo2p–RNAcomplex is in part composed of the RNA processing body (P-body)based on coprecipitation with P-body protein subunits and partialcolocalization of Myo2p with P-bodies. P-body disassembly isdelayed in the motor mutant, myo2-66, indicating that Myo2pmay facilitate the release of mRNAs from the P-body..