Oleynikov & Singer (2003): Figure 2

Supplementary Material From Singer Lab Publications:

Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization

Curr Biol 13(3):199-207 (2003 February 4)

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Figure 2. ZBP1 Associates with the Cytoskeleton

(A) Immunofluorescence staining of ZBP1 (red), actin (green), and tubulin (blue). Arrows indicate localization of ZBP1 along microtubules.

(B) Immunofluorescence staining of ZBP1 (red) and actin (green). Arrows indicate ZBP1 along filament bundles.

(C) ZBP1 distribution in cytochalasin-treated cells.

(D) ZBP1 distribution in colchicine-treated cells.

(E) ZBP1 distribution in BDM-treated cells. See Movie 12 in the Supplementary Material.

(F) Electron microscopy of ZBP1 in CEFs. Thin-sectioned cells were stained with anti-ZBP1 antibody and secondary gold-conjugated antibody. MT, microtubule; MF, actin.

The scale bar in (B) represents 2 µm in (A) and (B), the scale bar in (C) represents 10 µm in (C)–(E), and the scale bar in (F) represents 200 nm.