Oleynikov & Singer (2003): Figure 4

Supplementary Material From Singer Lab Publications:

Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization

Curr Biol 13(3):199-207 (2003 February 4)

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Figure 4. Distribution of ZBP1 Determines Distribution of ß-actin mRNA

(A–C) Expression of ZBP1-GFP in C2C12 cells. ß-actin mRNA (red); ZBP1-GFP (green). C2C12 cells were transfected with ZBP1-GFP DNA, fixed, and ß-actin mRNA-stained via in situ hybridization. The expression of ZBP1-GFP in cells without endogenous ZBP1 causes the formation and polarized location of particles of ß-actin mRNA. Nontransfected C2C12 cells in the same field exhibit a diffuse RNA pattern. The scale bar represents 10 µm.

(D) Control, just CFP fused to the membrane signal.

(E and F) Expression of ZBP1 fused to the neuromodulin membrane-targeting signal (green) accumulates at cellular membranes and mislocalizes ß-actin mRNA (red). The scale bar represents 5 µm.