Movie 1. Movement of GFP-ZBP1 in a Fibroblast Stimulated with Serum
Fibroblasts were grown overnight in serum-free conditions and were stimulated with serum while visualization was in process. Images were taken at 400-ms intervals for 310 s. (see Figure 1D and Movies 2-11 below)

Movie 12. GFP-ZBP1 Movement in Cells Treated with BDM
The particles do not translocate significantly. (see Figure 2E)

Movie 13. Recovery from Fluorescence Photobleaching of the ZBP-GFP1 Associated with the Transcription Site
The photobleached sample was then observed in the confocal microscope at a frame rate of one per 6.25 s for 175 s. The fluorescence was quantitated by using NIH Image and was plotted as the percent recovery. (see Figure 6C)

Movie 14. Movement of GFP-ZBP1 into the Nucleus
The particle jumps into the nucleus, as indicated by the arrow in Figure 6E. The frame capture rate is 800 ms.

Movie 2. Deconvolved Version of Movie 1
Huygens II Professional (SVI) software was used for two-dimensional deconvolution. (see Figure 1D, Movie 1 above and Movies 3-11 below)

Movies 3-11. Tracking of Individual Particles from Movie 2
Shown are the trajectories over time of 9 particles tracked in Movie 2. Numerical results are shown in the Supplementary Materials. (see Figure 1D, Movies 1 & 2 above and Figures S1-S9)