Supplementary Material From Singer Lab Publications:

Fig 1.  Alignment of deduced amino acid sequences of selected members of the PGM superfamily: PRP1 - Toxoplasma gondii parafusin-related protein (accession number AF295534), PFUS - Paramecium parafusin (accession number L12471), RBMPGM - Rabbit muscle PGM (accession number P00949).  Deletions and insertions present both in PFUS and PRP1, as compared to RBMPGM, are denoted by D# and I#, respectively; while deletions and insertions in PRP1 only are denoted by D#' and I#' and in Paramecium only by (D#) and (I#).  Comparison of the I-2 region of Paramecium PFUS with T. gondii PRP1 is shown in bold.  The underlined residues in PRP1 indicate the identical (uppercase) or highly conserved amino acids (lowercase).  A potential myristoylation site at the second residue is indicated by an asterisk.  Two regions important for PGM enzymatic activity are shaded gray: the active site, that has the serine boxed black, and the Mg²⁺-binding site.

Table 1: PRP1 identity and similarity to Paramecium PFUS and RBPGM