Fig 2. Southern
and Northern blots of PRP1.
(A)
The Southern blots indicating the presence of a single copy-gene.
A probe ranging from 373-1911 bp of the coding sequence of the PRP1
gene hybridized of Sac II and Kpn I-digested genomic DNA to single bands
as expected from the cDNA sequence.
(B)
The Northern blot shows that the gene is expressed. The PRP1
gene is expressed in both dividing (Div.) and isolated T. gondii
tachyzoites (Iso), but not in the parasite host cells (HFF). The
same probe as in (A) was used for the hybridization.
The
arrows and numbers indicate the size of the DNA fragments in kbp or
kilo bases, respectively. |
