Fig 7.
3-D reconstruction
of deconvolved images. Image volumes of cells A and D of Fig.
6 shown from five perspectives. Left panels: conventional
right-handed axis (longitudinal view).
Upper
panel: Image volumes of the cell shown in panel A of Fig. 6.
At no point are there voxel overlaps between micronemes (MIC3 antibody,
green channel) and rhoptries (ROP2 antibody, red channel),
demonstrating clear separation of the two types of fluorescently labeled
organelles.
Lower
panel: The thin yellow cap that fills most of the apex of the cell
represents voxel overlap between PRP1 (I-2 antibody, red channel)
and micronemes (MIC3 antibody, green channel). PRP1 co-localizes
principally with micronemes.
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