Matthiesen, et al (2001): Movies from Figure 6

Supplementary Material From Singer Lab Publications:

A Parafusin-Related Toxoplasma Protein in Ca2+-Regulated Secretory Organelles

Eur J Cell Biol 80(12):775-783 (2001 December)

Link to Journal | Download Reprint (PDF) | Figures & Table | Movies From Figure 6

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Fig 6 Movies. PRP1 co-localizes principally with micronemes.

3-D reconstruction of deconvolved fluorescence light microscope images of isolated, double-labeled tachyzoites. Overlap shown in yellow. The methodology separates two closely positioned organelles.

(A) Micronemes (MIC3 antibody) green and rhoptries (ROP2 antibody) red demonstrate organelle separation.

(B) Dense granules (GRA1 antibody) green and PRP1 (I-2 antibody) red.

(D) Micronemes (MIC3 antibody) green and PRP1 (I-2 antibody) red show co-localization.

Each image consists of multiple superimposed translucent planes, (A) 24 planes, (B) 25 planes, (C) 20 planes and (D) 21 planes, with a z-value of 100 nm. The nucleus is marked in blue (DAPI) and not deconvolved. The scale bar represents 2 mm.