Shav-Tal, et al (2006): Figure 1

Supplementary Material From Singer Lab Publications:

Gene expression within a dynamic nuclear landscape

EMBO J 25(15):3469-3479 (2006 August)

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Figure 1. Measuring histone mobility by FRAP analysis. Different kinetic populations of histone-GFPs are revealed by FRAP. (A) A small area within the nucleus of a cell expressing histone-GFP was bleached, and confocal images were collected every 10 min for 1 h and every 30 min thereafter, in order to follow recovery of the signal within the bleached region. (B–D) Relative intensities (±s.d.; n=9–22) within bleached areas were measured using images like those in (A). In some cases, the transcriptional inhibitor DRB (100 nM) was added 30–60 min before bleaching. Adapted and reprinted by permission from the Rockefeller University Press: Journal of Cell Biology (Kimura and Cook, 2001), copyright 2001.