Shav-Tal, et al (2006): Figure 2

Supplementary Material From Singer Lab Publications:

Gene expression within a dynamic nuclear landscape

EMBO J 25(15):3469-3479 (2006 August)

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Figure 2. Measuring RNA movement by photoactivation. A DNA locus (detected in red by transfection of an RFP-lac repressor protein) that transcribes a tagged mRNA was co-transfected with photoactivatable MS2-GFP (MS2-paGFP) in order to fluorescently tag the mRNA. Transcription from this gene was induced for 30 min by doxycycline. The locus was detected (red) prior to photoactivation (A), and the image in GFP before activation was recorded (B). The 405-nm laser was directed at the boxed region of interest (yellow), and the MS2-paGFP was detected at the transcription site 1.635 s after activation (C). Bar, 2 µm. (D) The RNA signal emanating from the transcription site was followed for 262 s (bar, 2 µm). Adapted and reprinted from by permission from the American Association for the Advancement of Science: Science (Shav-Tal et al, 2004a), copyright 2004.