Movie
1. Movement of tagged chromatin in yeast.
Lac operator repeats are tagged and allow the tracking of DNA in a yeast nucleus.
Adapted from (Heun et al., 2001) – Movie 2.
Movie 2. Dynamics of chromosome territories.
Live-cell imaging of a HeLa H2B-GFP nucleus that was replication-labeled in two consecutive S-phases with Cy3-dUTP (displayed red) and Cy5-dUTP (displayed green), respectively. Observation started 4.5 d after the second labeling event. The GFP signal is indicated by a gray outline. Inset shows the GFP signal of the corresponding confocal midsection at a lower magnification. In contrast to Cy3, Cy5 signals showed notable fading due to photobleaching; hence, signal contrast was digitally enhanced for compensation. Differentially labeled chromosome territories are displayed as maximum intensity projections from light optical serial sections recorded with a 10-min interval. For observation of intranuclear chromatin movements, images were corrected for translational and rotational nuclear movements. Movements of labeled chromatin are locally constrained, and red- and green-colored chromatin domains remain separated. The large-scale topography of labeled chromatin is maintained when the cell enters prophase 3 h and 50 min after the start of the observation period.
Adapted from (Walter et al., 2003) – Movie 2.
Movie 3. Tracking of mRNPs dynamics in the nucleus.
YFP-MS2 tagged mRNPs can be seen during release from the site of transcription in the center of the nucleus. mRNPs travel by diffusion through the nucleoplasmic space.
Adapted from (Shav-Tal et al., 2004a) – Movie 10.
Movie 4. Dynamic localization of the SR protein YFP-SF2/ASF from telophase to G1.
YFP-SF2/ASF localization was followed by confocal microscopy during telophase in living cells. YFP-SF2/ASF transiently accumulates in bright NAPs that persist for 15-20 min, and then disappear as YFP-SF2/ASF localizes to nuclear speckles in G1. Single optical sections were collected every minute for 100 min.
Adapted from (Bubulya et al., 2004) – Movie 2.
Movie 5. Cajal body dynamics.
A HeLaGFP-coilin cell transfected with histone H2B-YFP (red). GFP-coilin signal is in green and the movement of Cajal bodies is followed over time. Each frame is a maximum intensity projection and is separated by 3 minutes.
Adapted from (Platani et al., 2002) – Movie 1.
Movie 6. Movement of nuclei in Neurospora crassa.
High magnification movie of Neurospora crassa expressing H1-GFP localized in nuclei, co-labeled with the membrane selective dye FM4-64. Note the anterograde and retrograde movement of nuclei (hyphal tip to the right). Also note the prominent H1-GFP localization at the tips of pear shaped nuclei in transport. Period of time over which nuclei were imaged: 2 min.
Adapted from (Freitag et al., 2004) – Movie 2
