Rodriguez, et al (2006): Figure 2

Supplementary Material From Singer Lab Publications:

Visualization of mRNA translation in living cells

J Cell Biol 175(1):67-76 (2006 October 9)

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Figure 2. ReAsH labeling reveals the sites of β-actin translation. Epifluorescence images of C2C12 cells containing the FL (B–D) or ΔZIP (F–H) construct stained with ReAsH. (A) Schematic representation of the time course of the procedure used in B–D. (E) Schematic representation of the time course of the procedure used in F–H. (B and F) Epifluorescence image showing the distribution of mature GFP–ß-actin with a close up of the boxed region shown in the inset. (C and G) Epifluorescence image showing numerous bright puncta in the red channel, representing the distribution of nascent chains of ß-actin throughout the cell. (D and H) Overlay of the images, showing the proximity between the red nascent chains and green actin filaments.