Figure 8. N-cadherin is lost from adherens junction when the translation of β-actin is mislocalized.
(A) Indirect immunofluorescence image of N-cadherin staining of a cluster of C2C12 cells. The arrowheads point to the junction between a cell transfected with the ΔZIP or an FL construct. The arrows point to junctions between normal nontransfected cells. (B) N-cadherin staining of a cluster of C2C12 cells transfected with the FL transgene (arrowhead). (C) Differential interference contrast (DIC) image of the field shown in A. (D) Differential interference contrast image of the field shown in B. (E) Freehand regions of interest were drawn around the N-cadherin staining, and the mean fluorescence intensity of N-cadherin staining was determined using IPlab software. Quantification of the relative strength of N-cadherin staining was determined by comparing the ratio of the mean fluorescence intensity of N-cadherin at the junction with the mean fluorescence intensity of N-cadherin at the junctions between untransfected cells. The blue bar represents the ratio determined for cells containing the ΔZIP construct. The red bar represents the ratio determined for cells containing the FL construct. Eight fields of cells were compared for each construct. P = 0.005. Error bars are ± SEM.