Rodriguez, et al (2006): Figure S1

Supplementary Material From Singer Lab Publications:

Visualization of mRNA translation in living cells

J Cell Biol 175(1):67-76 (2006 October 9)

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	Index Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure S1
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Figure S1. Translation sites are associated with the cytoskeleton. C2C12 cells treated with cycloheximide and then with 0.15 mg/ml saponin and stained with ReAsH show translation sites that are resistant to detergent extraction. (A) Image of a C2C12 cell stained with ReAsH and extracted with saponin, showing the mature detergent-resistant β-actin cytoskeleton with schematic representation of the time course of the procedure. (B) Close-up overlay image of the boxed region from A showing mature protein (green) and nascent chains (red). Notice the close association of nascent chains and mature actin filaments.