Tiruchinapalli, et al (2003): Figure 3

Supplementary Material From Singer Lab Publications:

Activity-Dependent Trafficking and Dynamic Localization of Zipcode Binding Protein 1 and beta-Actin mRNA in Dendrites and Spines of Hippocampal Neurons

J Neurosci 23(8):3251-3261 (2003 April 15)

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Figure 3. Western blot analysis of ZBP1 expression.

A, KCl treatment for 15 min did not have a statistically significant effect on ZBP1 levels (normalized band intensities from three experiments). Densitometric ratio of ZBP1 to ß-tubulin from the unstimulated neurons was scaled to 100%. ZBP1 levels from neurons treated with KCl, cycloheximide (CHX), KCl plus cycloheximide, or KCl plus APV showed <10% fluctuation in normalized intensities (which were not statistically significant).

B, One representative Western blot for ZBP1 and ß-tubulin (normalized control) is shown as an example.