Tiruchinapalli, et al (2003): Figure 6

Supplementary Material From Singer Lab Publications:

Activity-Dependent Trafficking and Dynamic Localization of Zipcode Binding Protein 1 and beta-Actin mRNA in Dendrites and Spines of Hippocampal Neurons

J Neurosci 23(8):3251-3261 (2003 April 15)

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Figure 6. KCl-induced localization of ß-actin mRNA granules.

A, Localization of ß-actin mRNA granules in dendrites of an unstimulated neuron.

B, Increased localization of ß-actin mRNA in dendrites 15 min after KCl stimulation.

C, Exposure to the NMDA receptor antagonist AP-5 reduced the localization of ß-actin mRNA in dendrites.

D, Inhibition of NMDA receptor activation with AP-5 in the presence of KCl did not impair the depolarization-induced localization of ß-actin mRNA in dendrites.

E, Quantitative analysis of mean fluorescence intensities from 45 neurons per treatment demonstrated a significant increase in ZBP1 levels after KCl treatment in proximal, middle, and distal dendritic regions. Treatment of AP-5 alone significantly decreased the localization of ß-actin mRNA as compared with control unstimulated neurons but did not inhibit the KCl-induced increase in ß-actin mRNA. Bars show group means ± SEM; p <=0.05*, two-tailed Mann-Whitney t test. Black asterisks denote significance as compared with control untreated neurons. Red asterisks denote comparison of AP-5- and AP-5+KCl-treated neurons.