Zhang, et al (2003): Figure 1

Supplementary Material From Singer Lab Publications:

Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization

J Neurosci 23(16):6627-6637 (2003 July 23)

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Figure 1. Western blot analysis of SMN expression from rat spinal cord and chick forebrain at different embryonic stages.

A, In rat spinal cord, SMN expression was prominent at embryonic stages and declined developmentally.

B, In chick forebrain, SMN was also highly expressed at embryonic stages and declined after birth.

A, B, Density ratio of SMN to ß-actin is shown in the top panel. SMN was detected with a monoclonal antibody and showed a single band at 38 kDa. ß-actin was detected with a monoclonal antibody and showed a single band at 42 kDa, blotted on the same membrane as an internal control.