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| Index Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Table 1 | ||
| click
image for larger view (view larger image
at 100% magnification for best clarity) |
 |
Figure 5. EGFP-SMN accumulates in the nucleus after deletion of exon-7. SMN constructs, fused to EGFP, were transfected into cultured chick forebrain neurons to investigate the role of specific exons in subcellular distribution. Left panels, Imaged at 0.05 sec exposure times to show nuclear and perinuclear signal. Right panels, Imaged at 0.5 sec exposure time to show fluorescence signal in processes. Nuclei were stained with DAPI (red). A, Transfection of full-length EGFP-SMN revealed numerous granules in the perinuclear region. In the nucleus, one or two foci were often observed. B, In the same cell at longer exposure, granules were apparent in the processes. C, Exon-5 truncation (EGFP-SMNΔEx5) demonstrated a similar pattern to the full-length protein. SMN granules were observed in the perinuclear region with little signal in the nucleus. D, In the same cell, SMN granules were frequent in the processes. E, F, By contrast, deletion of exon-7 resulted in exclusive nuclear localization (EGFP-SMNΔEx7) with no signal in the perinuclear region (E) or processes (F). G, H, Deletions of both exon-5 and 7 (EGFP-SMNΔEx5&7) also showed an exclusive nuclear localization. |
