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| Index Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Table 1 | ||
| click
image for larger view (view larger image
at 100% magnification for best clarity) |
 |
Figure 6. Nuclear aggregates in the SMN exon-7 deletion mutant were not associated with coilin. A, An EGFP-SMN transfected neuron showed a single nuclear foci (arrowhead). In the cytoplasm, EGFP-SMN granules were observed in processes (arrows). B, A coiled body (red) was detected with an anti-coilin antibody in the nucleus. C, EGFP-SMN foci colocalized to a coiled body in an overlay image (yellow, arrowhead). D, DAPI stained nucleus (blue). E, By contrast, an EGFP-SMNΔEx7 transfected neuron showed nuclear aggregation with several foci (arrows). (see also video 2). F, Coiled bodies (red) were detected in the nucleus. G, Overlay of images indicated two foci of EGFP-SMNΔEx7 association with coiled bodies. H, DAPI stained nucleus of the same neuron (blue). |
