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| Index Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Table 1 | ||
| click
image for larger view (view larger image
at 100% magnification for best clarity) |
 |
Figure 8. Redistribution of nuclear EGFP-SMNΔEx7 by cytoplasmic targeting sequences. A, Fusion of the QNQKE sequence to EGFP-DBF1 (EGFP-DBF1/tif) revealed signal in the cell body (arrows), but not in a granular pattern in neurites (arrow). No signal was observed within distal neurites. B, Fusion of the QNQKE sequence to EGFP-SMNΔEx7 (EGFP-SMNΔEx7/tif) resulted in the presence of granules within the cell body and processes (arrows). C, Fusion of the membrane targeting sequence of GAP-43 to EGFP-SMNΔEx7 (mem/EGFP-SMNΔEx7) resulted in its redistribution into the cytoplasm and localization of granules in neurites (arrows; see video 3 for demonstration of its active transport in axons and video 4 for example of granule transport from the cell body into proximal neurite). Only weak signal from the mem/EGFP-SMNΔEx7 was observed in the nucleus. D, The GAP-43 targeting sequence also promoted EGFP-DBF1 localization in the cytoplasm (arrow). A-D, DAPI stained nuclei (red), approximate location of nuclear border (arrowhead). |
