SingerLab Online - From The Research Laboratory of Robert H Singer, PhD - Studying the Birth & Travels of RNA
Home Introduction People Publications Patents Protocols Contact Information


Supplementary Material From Singer Lab Publications:

 
Localization of ASH1 mRNA Particles in Living Yeast
Mol Cell 2(4):437-445 (1998 October)
Link to Journal | Download Reprint (PDF) | Figures | Movie From Figure 4 | About The Cover
 
  About The Cover:  
 
click image for larger view (view larger image at 100% magnification for best clarity)
 
 

The cover shows live yeast cells expressing two plasmids: one a fusion between green fluorescent protein containing a nuclear localization signal and the RNA-binding protein, MS2, and the other a reporter mRNA containing the MS2 RNA-binding sites with a 3' untranslated region from the ASH1 mRNA, sufficient for localization to the bud. The complex of the protein with the mRNA carries the GFP fusion out of the nucleus, where it forms green particles that localize in the bud.

 
 
contact the web designer   (c) copyright Yeshiva University