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Supplementary Material From Singer Lab Publications:

 
Gene expression profiling in single cells within tissue
Paola Capodieci, Michael Donovan, Heidi Buchinsky, Yusuf Jeffers, Carlos Cordon-Cardo, William Gerald, Jon Edelson, Shailesh M ShenoyRobert H Singer
Nat Methods 2(9):663-665 (2005 September)
 
 
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Methods
 
 
  Abstract:  
 
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
 
 
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