Figure 1.  Detection of nascent RNA (transcription sites) in paraffin-embedded tissue.  

(a) Detection of SMG1 (8 probes) transcription sites (arrows) in human PCa. Bar, 5 µm. (b) Detection of SMG1 (41 probes) nascent transcripts (arrows) in human PCa. Bar, 5 µm. (c) Colocalization of a gene and the respective chromosome. Prostate carcinoma exhibiting the colocalization of a DNA locus−specific probe for AR (androgen receptor, red spots) with the AR peT-FISH on a single section (green spots). Bar, 10 µm. (d) Multiplex detection of five genes in a prostate cancer sample (1982 archive), demonstrating restricted expression of four genes to the epithelial cells of the tumor annotated with the automated transcription site finder algorithm. The fifth gene in the barcoding scheme, EPB49, was only rarely detected and was not identified in this particular field. Bar, 10 µm.