Zhang, et al (2001): Figure 3

Supplementary Material From Singer Lab Publications:

Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility

Neuron 31(2):261-275 (2001 August 2)

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Figure 3. Localization of ZBP1 and ß-Actin mRNA Granules within Neuronal Processes and Growth Cones

(A) ZBP1 (green) and ß-actin mRNA (red) were localized within granules within neuronal processes and growth cones (top panel). Higher magnification of three regions (a, b, and c) showed colocalization of ZBP1 and ß-actin mRNA within numerous granules (arrows). Phase optics are shown in lower panel.

(B) Neurons were transfected with EGFP-ZBP1 and fixed for in situ hybridization to ß-actin mRNA (red). The overlaid images showed the colocalization or clustering of EGFP-ZBP1 with ß-actin mRNA in the process and growth cone (arrows). EGFP-ZBP1 was also observed at sites that lacked ß-actin mRNA signal (white arrowhead), as well as ß-actin mRNA granules which had no apparent staining for EGFP-ZBP1 (black arrow). Right panel: overlay on DIC optics.

(C) ZBP1 granules delineate several microtubules, labeled with anti-tubulin (green), within the central domain of the growth cone (arrowheads in enlarged inset). Right panel: DIC optics.