Zhang, et al (2001): Figure 6

Supplementary Material From Singer Lab Publications:

Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility

Neuron 31(2):261-275 (2001 August 2)

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Figure 6. Effects of Zipcode Antisense on EGFP-ZBP1 Granule Movements into Photobleached Neurites Following NT-3 Stimulation

Cells were pretreated with either control (A–D) or antisense (E–H) oligonucleotides to the ß-actin zipcode. Distribution of EGFP: (A and E) before photobleaching in a neurite that extends from cell body (inset), (B and F) immediately after photobleaching (t = 0), (C and G) 60 s, (D and H) 300 s. The neurite from the control treatment shows a rapid, almost full recovery of fluorescence whereas the neurite from the antisense treatment shows a slow, incomplete recovery of fluorescence. Granules moving into the neurite translocate more distal (d) in the control compared to the antisense, where they remain more proximal (p) to the cell body. (I) Time course of EGFP-ZBP1 translocation in either control (triangles) or antisense (squares) oligonucleotide-treated neurons.