Zhang, et al (2001): Figure 7

Supplementary Material From Singer Lab Publications:

Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility

Neuron 31(2):261-275 (2001 August 2)

Link to Journal | Download Reprint (PDF) | Figures | Movies

	Index Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8
	click image for larger view (view larger image at 100% magnification for best clarity)

Figure 7. Concentration of ß-Actin Protein within Growth Cones Following NT-3 Stimulation Is Dependent on Sequences within the ß-Actin 3'UTR

(A) Neurons were starved for 3 hr and treated with NT-3 in the presence of antisense or control oligonucleotides for 90 min and then stained for ß-actin by immunofluorescence. Antisense treatment inhibited the enrichment of ß-actin protein within growth cones whereas the control treatment resulted in a 75% increase in the fluorescence intensity for ß-actin protein (Student's t test: p < 0.01 when “control” group was compared to “control/NT-3” group).

(B) Neurons were transfected with ß-actin-EGFP fusion constructs, with and without the ß-actin mRNA 3'UTR, and then starved for 3 hr and treated with NT-3 for 90 min. Higher levels of ß-actin-EGFP fluorescent intensity were observed within growth cones from the construct that contained the ß-actin 3'UTR compared to the construct without the 3'UTR (p < 0.01).