Femino, et al (1998): About The Cover

Supplementary Material From Singer Lab Publications:

Visualization of Single RNA Transcripts in Situ

Science 280(5363):585-590 (1998 April 24)

	About The Cover:
	click image for larger view (view larger image at 100% magnification for best clarity)

A modification of fluorescence in situ hybridization allowed the detection of the birth of individual actin messenger RNAs (mRNAs), here in a digital image. Within the nucleus (delineated by the blue border), the gene is templating nascent RNAs, represented by the cluster of cubes (voxels). After synthesis, single mRNA molecules spiral away from this transcription site toward the nuclear periphery and move into the cytoplasm.

This cover image was rendered from a three-dimensional image volume that was captured and processed using the methods described in this publication. The image (right), which is from figure 4 (panel E, rotated 180 degrees) in this publication, is a two-dimensional image of the spiral shown on the Science cover.