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Press Release |
| Abstract: | ||
A
key goal of biology is to relate the expression of specific genes to a
particular cellular phenotype. However, current assays for gene expression
destroy the structural context. By combining advances in computational
fluorescence microscopy with multiplex probe design, we devised technology
in which the expression of many genes can be visualized simultaneously
inside single cells with high spatial and temporal resolution. Analysis
of 11 genes in serum-stimulated cultured cells revealed unique patterns
of gene expression within individual cells. Using the nucleus as the substrate
for parallel gene analysis, we provide a platform for the fusion of genomics
and cell biology: "cellular genomics." |
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