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Fig.
3. Population statistics from single cells. (A) Scatterplot
showing the correlation between nuclear cross-sectional area and number
of detected transcription sites in 181 DLD-1 nuclei. The linear
trend shows that for each additional 11.9 µm2 of
nuclear area, another transcription site is detected (r2
value, 0.56). (B) Histogram of the distribution of genes and
alleles in a population of expressing cells. For 10 genes assayed,
the highest occurrences were five genes and six alleles. Cells show
considerable heterogeneity in their gene patterns. (C) High and
low levels of transcription activity as measured by transcription site
detection. The g-actin, TIEG, and EGR-1 genes
showed a similar pattern of activation, with about 35% of cells with
one site and 35% of cells with both alleles. In contrast, the less robustly
transcribed species c-myc, cyclin D1, and MCL-1 showed lower levels,
with roughly 15% of cells containing a single site and fewer than 5%
with two. |
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