Fig. S4. Changes in temperature have an effect on diffusion coefficients. (A) Plot of fluorescent recovery after photobleaching (FRAP) of free nuclear YFP-MS2 coat protein. Diffusion coefficients were calculated from the fit of these curves. FRAP experiments were performed at 37°C, (B) 32°C, (C) 27°C, (D) 22°C. (E) FRAP of nuclear mRNPs in cells co-transfected with pTet-On and YFP-MS2 and induced for 30 to 60 min with doxycycline. Experiments were performed at 37°C, (F) 32°C, (G) 27°C, (H) 22°C. Diffusion coefficients (D) and immobile fractions (gamma) are indicated. (I) Temperature-dependent change in diffusion coefficients for free YFP-MS2 and (J) mRNPs. The changes in nuclear viscosities are indicated. (K) Comparison of the recovery curves and diffusion coefficients of free YFP-NLS and YFP-MS2.