Cell co-transfected with CFP-lac repressor, YFP-MS2 and pTet-On plasmids after 2 hour induction with doxycyline.

Movie S1. Detection of open gene locus by CFP-lac repressor.
Movie S2. Detection of YFP-MS2 nuclear mRNPs and YFP-MS2 accumulation at the transcription site.
Movie S3. Different threshold of same cell showing cytoplasmic YFP-MS2 mRNPs.
Movie S4. Detection of cytoplasmic CFP-peroxisomes (different plane than Movie S1).

Movie S5. Three-dimensional presentation of a fixed cell co-transfected with RFP-lac repressor, YFP-MS2 and pTet-On plasmids after 2 hour induction with doxycyline. The gene locus can be seen in red, the YFP-MS2 mRNPs in green and the CFP-peroxisomes in blue.

Cell co-transfected with CFP-lac repressor, YFP-MS2 and pTet-On plasmids after 20 minute induction with doxycyline, showing low levels of mRNPs and used for SPT.

Movie S6. YFP-MS2 mRNPs moving in the nucleoplasm. The gene locus is marked in red.
Movie S7. Track of diffusive particle in this cell.
Movie S8. Track of a corralled particle in this cell.

Cell co-transfected with CFP-lac repressor, YFP-MS2 and pTet-On plasmids after 30 minute induction with doxycyline.

Movie S9. Gene locus detected by CFP-lac repressor protein
Movie S10. Release of particle from transcription site.

Movie S11. Cell stably expressing pMS2-paGFP1 (2-3-6-PA) and co-transfected with RFP-lac repressor and pTet-On plasmids after 30 minute induction with doxycyline. The first three frames are a repeated frame before photoactivation. The transcription site was photoactivated using a 405 nm laser and the RNA signal at the transcription site and the nucleoplasm was followed. The nuclear envelope is marked in red using an image of full activation of the cell after the acquisition of the movie.

Cell co-transfected with YFP-MS2 and pTet-On. After 30 min activation with doxycycline Hochest was added to medium. Movies are from the cell after ATP depletion treatment.

Movie S12. YFP-MS2 mRNPs aggregate in the nucleoplasm.
Movie S13. Hoechst DNA stain showing area of condensed chromatin.
Movie S14. Merge – Hoechst in red and particles in green.

Cell transfected with H2B-YFP and followed throughout energy depletion.

Movie S15. H2B-YFP nuclear distribution prior to energy depletion.
Movie S16. H2B-YFP nuclear distribution after energy depletion.
Movie S17. H2B-YFP nuclear distribution after washout of metabolic inhibitors.

Movie S18. Cell transfected with H2B-YFP was bleached in the boxed areas and was imaged before and after ATP depletion.

Cell co-transfected with H2B-YFP and CFP-SC35.

Movie S19. Before ATP depletion.
Movie S20. After ATP depletion.

Cell co-transfected with YFP-MS2, CFP-SC35 and pTet-On. Doxycycline was added for 30 min. Cells were imaged after ATP depletion was performed.

Movie S21. YFP-MS2 mRNPs aggregate in nucleoplasmic structures.
Movie S22. CFP-SC35.
Movie S23. Merge.